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. 2020 Nov 7;8(4):664. doi: 10.3390/vaccines8040664

Figure 3.

Figure 3

Growth in MDBK cells (a) MDBK cells uninfected or infected with nLSDV∆SOD-UCT, nLSDVSODis-UCT or nLSDV at an multiplicity of infection (MOI) of 0.015. Monochrome light inverted microscopic images were taken at time points as indicated; (b) growth curves of nLSDV (red), nLSDVSODis-UCT (green) and nLSDV∆SOD-UCT (blue) in MDBK cells. Cells were infected at a MOI of 0.015 in triplicate and lysates were prepared 2 h post infection and at subsequent 1 day intervals. Titrations were performed to determine the TCID50 in MDBK cells; titers were converted to focus forming units per ml (ffu/mL). Data were analyzed by one-way ANOVA and post-hoc T-test. Data points denote mean and error bars SEM. ** denotes significant difference between nLSDV and nLSDV∆SOD-UCT at p < 0.01; ++ denotes significant difference between nLSDV∆SOD-UCT and nLSDVSODis-UCT at p < 0.01.