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. 2020 Oct 28;8(11):1671. doi: 10.3390/microorganisms8111671

Table 1.

Primers and Probes Designed for Duplex qPCR Assays accordingly to Species Examined.

Assay Name Sequences Names Sequences Specificity
CanFil-C. grassii C. Fil.354f GATCGTAATTTTARTACYTCTTTTTATGA Filaria/C. grassii
Can-fil.411p 6VIC-TATCAGCATTTGTTTTGGTTTTT-TAMRA
C. grassii.433p 6FAM-GGAAGGGTGGTAATCCTCTTCTTT-TAMRA
C. Fil.564r CAGCAATCCAAATAGAAGCAAA
C. bainae-C.spII T-Fil-62f TTGTCTTTTTGGTTTACTTTTGTGG C. bainae/Cercopithifilaria sp. II
C. bai.121p 6FAM-AGGGGGTGCTGGTAGCAGG-TAMRA
C. sp. II.116p 6VIC-GTTGGTAGAGGCCCTGGGAGT-TAMRA
T-Fil-337r GAAGTCAAATAAGAAGTRCAAACAAACA