Table 2.
In-vitro validation of the newly customized duplex qPCRs trough the DNA panel of filarioids and negative hosts (dogs and ticks).
| Panels of Tested DNA from Filarioids | CanFil-C. grasii | C. bainae-C. sp II | ||||
|---|---|---|---|---|---|---|
| Species Name | Specimens | Tested Samples (n) | Filarial DNA | C. grassii | C. bainae | C. sp. II |
| C. bainae | Adult worms | 1 | 1 | Negative | 1 | Negative |
| Larva F1 “Microfilaria” | 2 | 2 | Negative | 2 | Negative | |
| Infected ticks | 5 | 5 | Negative | 5 | Negative | |
| Cercopithifilaria sp. II | Larva F1 “Microfilaria” | 1 | 1 | Negative | Negative | 1 |
| C. bainae + C. sp. II | Mixed DNA | 3 | 3 | Negative | 3 | 3 |
| C. grassii | Larva F1 “Microfilaria” | 1 | 1 | 1 | Negative | Negative |
| C. grassii + C. bainae | Mixed DNA | 3 | 3 | 3 | 3 | Negative |
| C. grassii + C. sp. II | Mixed DNA | 2 | 2 | 2 | Negative | 2 |
| O. lupi | Larva F1 “Microfilaria” | 1 | 1 | Negative | Negative | Negative |
| Infected skin | 6 | 6 | Negative | Negative | Negative | |
| D. immitis | Adult worms | 2 | 2 | Negative | Negative | Negative |
| D. repens | Adult worms | 1 | 1 | Negative | Negative | Negative |
| A. reconditum | Blood microfilaria | 1 | 1 | Negative | Negative | Negative |
| T. callipaeda | Adult worms | 2 | 2 | Negative | Negative | Negative |
| S. digitata | Adult worms | 2 | 2 | Negative | Negative | Negative |
| Mansonella sp. | Blood microfilaria | 1 | 1 | Negative | Negative | Negative |
| M. perstens | Blood microfilaria | 1 | 1 | Negative | Negative | Negative |
| W. bancrofiti | Blood microfilaria | 2 | 2 | Negative | Negative | Negative |
| Loa loa | Blood microfilaria | 1 | 1 | Negative | Negative | Negative |
| Brugia sp. | Infected mosquitoes | 3 | 3 | Negative | Negative | Negative |
| B. malayi. | Infected mosquitoes | 3 | 3 | Negative | Negative | Negative |
| Filarial free samples | Ticks | 5 | Negative | Negative | Negative | Negative |
| Dog skin | 5 | Negative | Negative | Negative | Negative | |