Table 4.

Sensitivity and Assay Performance Characteristics of the Duplex COI-based System in Detecting D. immitis Microfilariae.

SQ mfs/mL	SQ Per qPCR Reaction from mfs/5µL	Ct Mean	E-RFU	SCRS
4.7 × 10+2	2.35 × 100	24.26	1554	[E = 96.9%] [S = −3.398] [Y.int = 33.2] [R2 = 0.998]
4.7 × 10+1	2.35 × 10−1	27.35	1363
4.7 × 100	2.35 × 10−2	31.03	1046
4.7 × 10−1	2.35 × 10−3	34.02	596
4.7 × 10−2	2.35 × 10−4	37.92	175
4.7 × 10−3	2.35 × 10−5	N/A	N/A
4.7 × 10−4	2.35 × 10−6	N/A	N/A
4.7 × 10−5	2.35 × 10−7	N/A	N/A
4.7 × 10−6	2.35 × 10−8	N/A	N/A
4.7 × 10−7	2.35 × 10−9	N/A	N/A
Cut Off Value		38.0	161
Negative Control		N/A	6.09

SQ: Starting Quantity, mfs: microfilaria, Cq: cycle quantification value; N/A: No amplification, E-RFU: End of relative fluorescence unit, SCRS: Standard Curve Results Spreadsheet, E: Efficiency, S: Slope, Y.int: Y-intercept.