Table 5.
Distribution of Positive Samples Detected by Each Molecular Assay (i.e., Multiplex qPCRs and PCR/Sequencing) from Canine Blood and Tick Samples.
| Filarial Species | Multiplex qPCR | PCR/Sequencing | ||
|---|---|---|---|---|
| Blood | Tick | Blood | Tick | |
| Single-species DNA | ||||
| D. immitis | 14 | 9 | 14 | 9 |
| D. repens | 2 | 2 | 2 | 2 |
| A. reconditum | 1 | 1 | 1 | 1 |
| C. bainae | 0 | 0 | 0 | 0 |
| Cercopithifilaria sp. II | 0 | 2 | 0 | 2 |
| Total | 17 | 14 | 17 | 14 |
| Multi-species DNA | ||||
| D. immitis and D. repens | 2 | 1 | 2 ur | 1 ur |
| D. immitis and A. reconditum | 3 | 3 | 3 ur | 3 ur |
| D. immitis and C. bainae | 0 | 4 | 0 | 4 ur |
| D. immitis and Cercopithifilaria sp. II | 0 | 1 | 0 | 1 ur |
| D. immitis, D. repens and C. bainae | 0 | 1 | 0 | 1 ur |
| Total | 5 | 10 | 5 ur | 10 ur |
ur: PCR positive samples that yielded unreliable DNA sequences.