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. 2020 Dec 3;10:21018. doi: 10.1038/s41598-020-78056-0

Figure 5.

Figure 5

PYK2-Src activation is required in TGF-β1 mediated CTGF upregulation. (A) Western blot of different phosphorylated forms of PYK2 as well as CTGF in LX2 pretreated with two SRC inhibitors (PP2 and Saracatinib) (n = 3). (B) qPCR of CTGF mRNA expression (shown as fold change, n = 3) and (C) Western blot of α-SMA in LX2 treated as indicated (n = 2). (D) Western blot of CTGF and different phosphorylated forms of PYK2 (n = 2) and (E) qPCR of CTGF, TGFB1, and SMAD7 mRNA expression (shown as fold change, n = 3) in LX2 transfected with siRNA of control or Src. Student's t-test; *p < 0.05, **p < 0.01, and ***p < 0.001.