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. 2020 Dec 3;10:21157. doi: 10.1038/s41598-020-78192-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Calbindin-D28k (CB) immunoreactivity in camel cerebellum. (a) CB immunoreactivity was obvious in almost all cerebellar cortical layers and the white matter (W). Purkinje cells (PC) showed the highest CB immunoreactivity. In addition, the dendrites extend the whole length of the molecular layer, showing an obvious dendritic arborization (D). (b) Seldom low CB-immunopositive PC bodies were observed (arrow). Neuron of Lugaro (L) and candelabrum neurons (CN) were observed. (c) CB immunoreactivity was seen as densely packed homogeneous deposits in the cytoplasm of the Purkinje cells and within the nuclei (N). Notice, CB immunoreactive fibers beneath and surrounding the PC, which are likely from basket and/or Lugaro cells (arrow). Immunoreactivity was observed in the spiny branchlets (arrowhead). (d) CB-immunopositive fibers (arrow) were traversing through the granular layer (G) and were oriented vertically till reaching the white matter. Inset showing the varicosities (arrowheads) along the axon profile which originated from the PC. (e) CB immunoreactivity was observed along the dendritic arborization till reaching the pial surface (P), arrowheads indicates the spiny branchlets of the dendrities.