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. 2020 Dec 3;10:21029. doi: 10.1038/s41598-020-78195-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sepsis is associated with increased extramitochondrial but diminished intramitochondrial TFAM abundance. Results representing PBMCs from sepsis patients (n = 10; blue bars) sampled within 24 h after the diagnosis of sepsis compared to healthy controls (n = 20; grey bars). Upper panel: Concentration of selected cytokines serum of septic patients and healthy controls. (a) TNF-α, (b) Interleukin-6, and (c) Interleukin-10. (d) Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) level (ELISA of nuclear protein extracts). (e) Relative TFAM mRNA expression (quantitative polymerase chain reaction; compared to beta actin) of PBMCs; AU: arbitrary units. (f,g) Relative TFAM protein expression in cytonucleoplasm normalized to beta actin (f) and relative intramitochondrial TFAM protein amount normalized to TNF receptor-associated protein 1 (g). Each circle represents an individual volunteer/patient; columns with error bars represent mean and SD. There were no missing data. P-values were determined using the Mann–Whitney test; *p < 0.05, **p < 0.01, ***p < 0.001. Cytokine concentrations pg/mL were derived from a calibration curve. All exact values are presented in the Source Data file.