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. 2020 Dec 3;10:21095. doi: 10.1038/s41598-020-77858-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of RORα deletion in macrophages on liver upon CDAA diet feeding. Ten weeks old WT and MKO mice were fed with either a chow or a CDAA diet for 8 weeks. (A) Representative liver gross morphology. (B) Liver weight. (C,D) Hematoxylin & Eosin staining of liver sections (C) and quantification of lipid droplets (D). Images were acquired on an Eclipse Ti-U microscope (Nikon) and quantified with Image J version 1.51j8 software (NIH, https://imagej.nih.gov/ij/). (E) Hepatic triglycerides (TG) content. (F) mRNA expression levels measured by RT-qPCR for Tnf, Il1b, Il6, and Il10 genes in liver. (G) Plasma transaminases activity. (H,I) Sirius red staining of liver sections (H) and quantification of fibrosis (I). Images were acquired on an Eclipse Ti-U microscope (Nikon) and quantified with Image J version 1.51j8 software (NIH, https://imagej.nih.gov/ij/). (J) mRNA expression levels measured by RT-qPCR for Col1a1, Col3a1, and Acta2 genes in the liver. Data are shown as mean ± SEM. *p < 0.05 by 2-way ANOVA followed by Sidak’s multiple comparisons test. All statistical analyses were carried out using GraphPad Prism 8 for Windows (GraphPad Software). n = 10–17 mice per group. NS: Not significant; FC: Fold Change. See also Fig. S4.