Table 1.
Strains and plasmids used in this study
| Strains or plasmids | Description | Source or reference |
|---|---|---|
| Strains | ||
| C48–1 | Avian P. multocida C48–1. Capsulated and virulent | China Veterinary Culture Collection Center |
| △aspA::kan | aspA mutant strain of Avian P. multocida C48–1 | This work |
| C△aspA::kan | Complemented strain of △aspA::kan | This work |
| △aspA::kan (pAL99S) | aspA mutant strain harboring an empty vector | This work |
| Plasmids | ||
| pBC-Tn903 | Suicide vector, CmR, KanR | [39] |
| pBC-aspA | Containing left and right arms of aspA, CmR | This work |
| pBC-aspA-kan | Constructing aspA gene deletion mutants, CmR, KanR | This work |
| pAL99S | P. multocida expression plasmid, derivative of pAL99, SpecR | [40] |
| pAL99S-aspA | Containing the intact aspA, SpecR | This work |
| pET-28a | Amplifing the kanamycin resistance cassette, KanR | Our Lab |
KanR kanamycin resistance, CmR chloramphenicol resistance, SpecR spectinomycin resistance