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. 2020 Dec 3;10:21015. doi: 10.1038/s41598-020-78058-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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5-Aza-2′deoxycytidine induced CDK9 recruitment and S2P RNAPII occupancy on the HEXIM1 promoter. C4-2 and LNCaP cells were treated with VE-822 (2 μM) or caffeine (2 μM) for 2 h, followed by 5-AzadC for 2 h. Cells were processed for ChIP analyses of the occupancy of CDK9 on the HEXIM1 promoter and occupancy of serine 2 phosphorylated (S2P) RNAPII on the HEXIM1 coding region. Input DNA was used as normalization control. IgG control was used as a negative control. Figures are representative of at least 3 independent experiments. *P < 0.05 and **P < 0.01 vs. Control.