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. 2020 Dec 3;10:21015. doi: 10.1038/s41598-020-78058-y

Figure 5.

Figure 5

HEXIM1 is required for 5-Aza-2′deoxycytidine-induced apoptosis and inhibition of cell proliferation by 5-Aza-2′deoxycytidine. (A) C4-2 cells were infected with control or HEXIM1shRNA lentiviruses followed by puromycin selection. Some cells were transfected with p53 expression vector and/or treated with 5-AzadC (5 μΜ, 48 h). Cell lysates were prepared and expression of indicated proteins were analyzed by western blot. Represented are blots cut into strips prior to blotting to minimize the amounts of antibodies required. Figures are representative of at least 3 independent experiments. *P < 0.05 and **P < 0.01, ***P < 0.001 versus Control, and #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. HEXIM1shRNA. (B) C4-2 cells were infected with control or HEXIM1shRNA-containing lentiviruses followed by selection using puromycin and plating for cell proliferation. Some cells were treated with 5-AzadC. After 5 days, WST-1 solution (10 mg/ml) was added to each well. The formazan crystals formed inside the viable cells were measured at 450 nm using a microplate reader. Figures are representative of at least 3 independent experiments **P < 0.01 vs. Control.