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. 2020 Dec 3;10:21015. doi: 10.1038/s41598-020-78058-y

Figure 6.

Figure 6

5-Aza-2′deoxycytidine-induced HEXIM1 expression in triple negative breast cancer is mediated by ATM and NF-ĸB, and HEXIM1 is critical for 5-Aza-2′deoxycytidine-induced apoptosis. (A) MDA-MB-231 and MDA-MB-468 cells were infected with control or NF-ĸB shRNA lentiviruses followed by puromycin selection or pre-treated with ATM inhibitor, caffeine (2 µM). Some cells were then treated with 5-AzadC (5 µM) for 48 h. (B) MDA-MB-231 and MDA-MB-468 cells were infected with control or ATM shRNA lentiviruses followed by puromycin selection. Some cells were then treated with 5-AzadC (5 µM) for 48 h. (C) MDA-MB-231 were infected with control or HEXIM1shRNA lentiviruses followed by puromycin selection. Some cells were treated with 5-AzadC (5 μΜ, 48 h). For (A),(B), and (C) cell lysates were prepared and expression of indicated proteins were analyzed by western blot. Represented are blots cut into strips prior to blotting to minimize the amounts of antibodies required. *P < 0.01 versus Control. Figures are representative of at least 3 independent experiments.