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There is no effect of Nec-1 on caspase-3 (A) or TUNEL (B) stained cells after retinal ischemia. Rats were pretreated with 4 mM Nec-1 or DMSO by an intravitreal injection at the time of and 2 hr after ischemia. TUNEL or caspase-3 staining was then performed after 24 hr of reperfusion. Cell counting in the GCL is shown. N = 4 per group.
