Table 1.

 Cell‐cycle distribution analysis of HeLa cells on a tissue culture dish, in microcapsules, and on agarose gel at indicated culture periods

	Culture condition	G0/G1 (%)	Synthetic (%)	G2/M (%)
2‐D	Log phase	56.3 ± 1.4	18.4 ± 0.9	25.1 ± 0.5
	Stationary phase	64.3 ± 1.9	19.2 ± 1.3	16.4 ± 2.7
Capsule	Day 6	63.4 ± 2.0	21.2 ± 0.2	15.4 ± 1.8
	Day 18	71.2 ± 0.9	15.5 ± 0.9	13.3 ± 1.7
	Day 18 → Agarose Day 3†	65.0 ± 0.9	23.9 ± 1.4	11.1 ± 0.9
Agarose	Day 9	65.3 ± 0.5	20.8 ± 1.0	13.9 ± 0.7

†After 18 days of culture in microcapsules, the spheres of cells were harvested and cultured on agarose gel for 3 days. Data are expressed as the mean ± SD (n = 3). 2‐D, cultured on a tissue culture dish; Agarose, cultured on agarose gel; Capsule, cultured in microcapsule.