Figure 5.

 Establishment of activation‐induced cytidine deaminase (AID)‐positive transfectants. The full‐length AID transcript was transfected into three AID‐negative cell lines established from different patients with rapidly progressive follicular lymphoma (FL; designated FL30 and FL32). Three clones from the FL30 patient and two clones from the FL32 patient were established. (A) Differences in the expression levels of AID among the transfectants were determined by quantitative real‐time PCR. (B) Western blot analysis showed that the AID‐transfectants expressed AID protein. (C) Immunohistochemical analysis showed that the transfectants expressed significant amounts of AID protein, predominantly in the cytoplasm. (D) Similar results were obtained for the transfectants established from FL32.