Table 1.

Assay Names, Molecular Methods and Targets, and Laboratory Testing Platforms

Assay	Manufacturer	Target(s)	Laboratory Test Platform
Assay 1	Xpert CT/NG Assay (Cepheid, Sunnyvale, California)	Real-time PCR to detect 2 noncontiguous chromosomal DNA regions from Neisseria gonorrhoeae (NG2 and NG4)—both of which must be positive to yield a positive result—and 1 chromosomal DNA target from Chlamydia trachomatis (CT1) [20]	GeneXpert System
Assay 2	Aptima Combo 2 Assay (Hologic, Inc, Marlborough, Massachusetts)	Utilizes target capture, TMA, and a dual kinetic assay to detect regions from the 16S rRNA of NG and the 23S rRNA from CT using labeled DNA probes [23]	Panther System
Assay 3	Abbott RealTime CT/GC assay (Abbott Laboratories, Abbott Park, Illinois)	A combination assay that uses real-time PCR to detect a highly conserved region within the Opa gene of NG and 2 distinct regions within the CT cryptic plasmid DNA [19]	Abbott m2000 RealTime System
NG tiebreaker	Aptima NG assay (Hologic, Inc)	Utilizes target capture, TMA, and hybridization protection assays to identify the presence of NG 16S rRNAa [20]	Tigris DTS System
CT tiebreaker	Aptima CT assay (Hologic, Inc)	Utilize target capture, TMA, and hybridization protection assays to identify the presence of CT 16S rRNAa [21]	Tigris DTS System

Abbreviations: CT, Chlamydia trachomatis; DTS, direct tube sampling; GC/NG, Neisseria gonorrhoeae; PCR, polymerase chain reaction; rRNA, ribosomal RNA; TMA, transcription-mediated amplification.

^aBoth tiebreaker assays utilize distinct molecular genetic targets from the assays under evaluation.