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. Author manuscript; available in PMC: 2021 Feb 13.
Published in final edited form as: Curr Top Dev Biol. 2020 Feb 13;137:279–305. doi: 10.1016/bs.ctdb.2019.11.010

Figure 2: Nodal and WNT signaling dynamics in BMP treated micropatterned hESC colonies.

Figure 2:

(A) Pie sections of a live-imaged micropatterned GFP-beta-catenin hESC colony stimulated with BMP4 showing a clockwise time evolution (17h to 47h) of non-membrane beta-catenin expression. Each section is a snapshot from time-lapse imaging at clockwise increasing time points post BMP treatment. The outer orange circumference highlights the pinned back end of the WNT wave while the white spiral approximates the inward movement of the front end of the WNT wave. Data from ref [71]. (B) Pie sections of micropatterned hESC colonies stimulated with BMP4 showing a clockwise time evolution (24h to 48h) of SMAD1 (red) and SMAD2 (cyan) expression. Each section corresponds to a micropatterned colony fixed at the indicated time point post BMP treatment. The red circumference highlights that SMAD1 stays restricted to the edge at all time points. The white spiral approximates the front end of the SMAD2 wave that moves inward in time. Data from ref. [69].