Figure 6. . Dextran sulfate sodium model of colitis has negligible effects on VPAC1 expression in the distal colon.

(A) Graphical representation of VPAC1 mRNA in the Distal colon. Gene expression normalized to internal control GAPDH. mRNA isolated from mouse distal colon was subjected to qPCR with specific primers for VPAC1. Data represented as average ± SEM, n = 6. (B) Immunofluorescence staining of VPAC1 (red) and DAPI (blue) in distal colonic tissue sections.

DAPI: 4,6-diamino-2-phenylindole; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; qPCR: Quantitative polymerase chain reaction.