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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Vaccine. 2019 Jul 13;37(35):4947–4955. doi: 10.1016/j.vaccine.2019.07.018

Fig. 1.

Fig. 1.

MDA expression and apoptosis induction after γ-irradiation. (A) mRNA levels for MDAs were assessed by qRT-PCR in B16-F1 and Cloudman cells. The 2−ΔΔCt values were calculated using B16-F1 and Cloudman Cts compared to H5V endothelial cells Cts. Actb was used as reference gene. Mean ± SD from two independent experiments is shown. (Studenťs t test, p > 0.05). (B) B16-F1 and Cloudman cells were γ-irradiated (70 Gy) and cultured for 0, 24 or 48 h. They were then stained with AnV-FITC and IP, and percentages of apoptotic/necrotic cells were assessed by flow cytometry.