TABLE 1.

Primer sequences, chloroplast loci, and GenBank numbers

No.	Primer pair	Sequence	Locus	GenBank numbers	Size
1	ACECP005	F: AAAATGGGGTTCCTAGTGGA R: ACTCGAACTCGAAGAAATGG	rps16 intron–5′‐rps16 CDS–IGS toward trnQ	KF284854–KF285047	548
2	ACECP016	F: TTTACAGTCCGTCCCCATTA R: CATCTCTCTTTCAAGGAGGC	trnY–IGS–trnE	KF285048–KF285088	139
3	ACECP018	F: AGAGAGATCTTGTTGATATTTGT R: TAGTCATGATTCAACGGGTC	IGS between trnT and psbD	KF284164–KF284369	254
4	ACECP026	F: ACTACGGTAGAGCGGTTTAT R: AAAGTCATCTCACGTTCACC	rbcL	KF284370–KF284574	402
5	ACECP035	F: TGGTTAGGTATTGGAGCAAC R: GTGGACATTCTACAGAAGCA	petD–IGS–rpoA	KF285089–KF285278	253
6	ACECP039	F: AGTTACTCCCTTTTCCACCA R: GTAATGTTGGGGTGAACCAA	IGS between rpl22 and rps19–rps19–IGS–rpl2	KF284575–KF284775	589

Forward and reverse primer sequences and locus information for the six chloroplast loci were analyzed. The ACECP prefix used in the name of each primer pair is an acronym for “Ahmed, Colocasia esculenta, chloroplast.” Last column indicates size (bp) in the final alignment of all sequences obtained for each locus (not the size of individual sequences). Individual GenBank accession numbers are listed in Table S2.