Fig. 4.

Effect of pramlintide on capillary tube formation on Geltrex™. HUVECs were seeded on Geltrex™ and treated without or with pramlintide (1 and 10 μg/mL) or VEGF (2 ng/mL) for 24 h. Quantitation of tube formation was done by calculating the average (A) tubules length, (B) tubules size, and (C) mean number of junctions. Data are shown as means ± SEM from triplicate experiments. ***P < 0.001 indicates significant differences compared to the control group (untreated cells); ^##P < 0.01 and ^###P < 0.001 versus VEGF group. HUVECs, human umbilical vein endothelial cells; VEGF, vascular endothelial growth factor.