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SDS-PAGE of the extracted proteins (Tobacco etch virus protease) in lysis buffers designed by RSM method to determine the best concentration of three additives chosen by Plackett-Burman method. T' and S' show total samples and soluble samples, respectively. Numbers correspond to runs in Table 5. BL21 cells that were not transformed with any plasmids were used as the negative control. SDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; RSM, response surface methodology.
