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Optimized lysis buffer designed by Plackett-Burman method. The concentration of variables is molar.
| Trehalose | Glycine betaine | Mannitol | Sodium citrate | Proline | L-Arginine | Xylitol | Sodium selenite | Dipotassium phosphate | CuCl2 | CTAB | Band intensity | Desirabilit |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.256 | 0 | 0 | 0 | 0.5 | 0 | 1 | 0.005 | 0 | 0.005 | 0.01 | 12692 | 0.848 |
