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Oral keratinocytes immortalized with Y‐27632 senesce after Y‐27632 withdrawal. (A–D) Both NOK805 (A, B) and D6 (C, D) cells senesce after removal of Y‐27632 from culture conditions, as shown by the increase in SA‐β‐Gal (A, C) activity and p16 expression (B, D). In the presence of Y‐27632, NOK805 and D6 cells were still proliferative at passage 18 and 17, respectively (A, C). Scale bars are 50 μm (N = 3 independent experiments).
