Fig. 4.

ATP increases the binding of HN to Aβ. Aβ40 (A, 100 nm) or Aβ42 (B, 100 nm), preincubated in a buffer in the absence or presence of 200 μm ATP, was bound to the wells, and then increasing concentrations of biotinylated‐HN were added and processed as described in Materials and methods. Optical density measurements (450 nm) were normalized by expressing each point in relation to the best‐fitted E _max value (set to 100%) and then plotted as a function of increasing biotinylated‐HN concentrations. The data were fit to a single binding site model with a nonlinear regression curve fitting approach using graphpad prism 8.4.3. Data were expressed as the mean ± SD of three independent experiments, each performed in triplicate.