Fig. 6.

Exogenously added ATP increases HN bound to Aβ from media of A549 and H1299 cells using 82E1 antibodies, resulting in decreased binding of exogenously added HN to Aβ. Conversely, less AChE is found in a complex with Aβ in the presence of added ATP in A549 cells. Anti‐Aβ‐specific antibodies (82E1) were added (1 : 1000 dilution) to ELISA plate wells. The wells were blocked, and 300 µL of conditioned media (0.5 µg·µL⁻¹) of A549 cells (A) or H1299 cells (B), 72 h after serum starvation, was added without or with ATP and increasing HN concentrations, and then, the HN and AChE bound were detected using the corresponding specific primary antibodies and processed as described in Materials and methods. Fold change relative to controls incubated with all components except the primary antibodies was calculated and fit, using the graphpad prism 8.4.3 software, with a nonlinear regression curve. The data represent the mean ± SD of three separate experiments, each performed in triplicate.