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. 2020 Nov 10;10(12):2761–2770. doi: 10.1002/2211-5463.13014

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© 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Effect of tofogliflozin administration on tubulointerstitial injury. (A) Immunohistochemistry analysis on (A) Masson's trichrome staining (20×, scale bar: 100 μm). Immunofluorescence analyses of the renal sections of each mouse with (B) KIM‐1 (green) and DAPI (blue) (20×, 60×, scale bar: 100, 20 μm). (C) F4/80 (40×, scale bar: 50 μm), (D) cleaved caspase 3 (40×, scale bar: 50 μm), and (E) TNFR2 (20×, scale bar: 100 μm) (n = 5 per group, at least 10 randomly selected renal sections per mouse). Data are presented as mean ± standard deviation. One‐way ANOVA was applied.