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. Author manuscript; available in PMC: 2020 Dec 3.
Published in final edited form as: Methods Mol Biol. 2015;1331:27–40. doi: 10.1007/978-1-4939-2874-3_3

Fig. 1. Glycan microarray binding assay.

Fig. 1

16 arrays are printed on a single slide with hundreds of BSA-modified neoglycoproteins on one array. Prior to the assay, the slide is fitted with a 16-well module that physically separates the individual arrays. In the binding assay, the slide is first blocked to deactivate reactive functional groups on surface. After blocking, it is incubated with sample of interest, and then the captured antibodies are detected with fluorophore-labeled secondary reagents. Binding is quantitated by a fluorescent scanner. In the example shown, IgG and IgM antibodies are detected with secondary reagents labeled with different dyes