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. 2020 Dec 3;15(12):e0230545. doi: 10.1371/journal.pone.0230545

Fig 1. Evaluation of in vivo infection of two L. infantum strains.

Fig 1

(A) Spleen weights of BALB/c and Swiss Webster mice infected by L. infantum strains. (B) Parasite load in the spleens of infected mice. (C) Nitrite levels (NOS activity) of spleen cell cultures. (D) Urea levels (ARG activity) of spleen cell cultures. BALB/c and Swiss Webster mouse infections were maintained for 30 or 60 dpi. The number of parasites/mg of tissue was estimated based on the total weight of the spleen removed and the parasite load in the serial dilution. The nitrite and urea levels were measured by spectrophotometry at 540 nm. Control: noninfected mice; LII (L. infantum infantum); LIC (L. infantum chagasi). *p ≤ 0.05; **p ≤ 0.0009; ***p < 0.0001. The values are represented by mean ± standard deviation of 3 independent experiments with 5 animals in each group.