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. 2020 Dec 3;15(12):e0230545. doi: 10.1371/journal.pone.0230545

Fig 2. In vitro proliferation of promastigote forms.

Fig 2

Promastigotes were obtained from amastigotes freshly isolated from the four infected experimental groups and cultivated for 7 days. The parasites were counted daily, and the growth profile was evaluated. LIC.B (L. infantum chagasi isolated from BALB/c mice); LII.B (L. infantum infantum isolated from BALB/c mice); LIC.S (L. infantum chagasi isolated from Swiss Webster mice); and LII.S (L. infantum infantum isolated from Swiss Webster). *p ≤ 0.05; **p ≤ 0.009; ***p < 0.0001. The values are represented by mean ± standard deviation of 3 independent experiments realized in experimental triplicate.