Table 1. Evaluation of the metacyclogenesis for the four Leishmania isolates.
| % metacyclic parasites | ||||
|---|---|---|---|---|
| Time | BALB/c | Swiss Webster | ||
| LII.B | LIC.B | LII.S | LIC.S | |
| 48h | 56.0 ± 5.7** | 27.0 ± 3.0 | 49.0 ± 7.8* | 22.0 ± 3.5 |
| 72h | 75.0 ± 3.5** | 38.0 ± 2.0 | 68.0 ± 4.2** | 34.0 ± 6.4 |
| 96h | 56.0 ± 1.4* | 31.0 ± 1.4 | 43.0 ± 6.4* | 18.0 ± 1.4 |
Percentage of metacyclic forms was determined by complement lysis test. LIC.B (L. infantum chagasi isolated from BALB/c mice); LII.B (L. infantum infantum isolated from BALB/c mice); LIC.S (L. infantum chagasi isolated from Swiss Webster mice); LII.S (L. infantum infantum isolated from Swiss Webster mice).
*p ≤ 0.0009;
**p < 0.0001. Comparison of metacyclic form percentages observed on LII and LIC isolates from each mouse lineage. The values are represented by mean ± standard deviation of 3 independent experiments realized in experimental triplicate.