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. 2020 Dec 3;14(12):e0008876. doi: 10.1371/journal.pntd.0008876

Table 2. HDR mediated CRISPR Cas9/sgRNA AeAct4 mutagenesis in Ae. aegypti.

Knock-out only (no HDR marker) HDR mediated knock-in
Injected components Injected G0 eggs Adult G0 survivors (%) G0 mosaic females (%) G0 founder pools flightless G1 females /total G1 females (%) G1 AeAct4 mutant flightless females (%) G0 founder pools G1 marker positive adults/total G1 (%)
AGG1070 AeAct4
donor plasmid,
Cas9 protein,
AeAct4 sgRNAs (1–4)		 600 97 (16.2) 0 (0) 5 8/3557 (0.2) 2/8 (25) 1 14/7198 (0.2)

AeAct4 inactivation by CRISPR/Cas9 mediated knock-in of a marker cassette. An initial fluorescence marker screen identified knock-ins from WT or knock-outs. sgRNA concentration was 40 ng/μl, Cas9 protein 400 ng/μl and donor plasmid 700 ng/μl.