Fig 5. Partial Na_v channel sequence with primers for kdr-like diagnostic PCR, partial Na_v amplification, and sequencing.

Intronic regions are in gray. The primer set of diagnostic PCR: control primers (ControlF and ControlR) are boxed in black; the diagnostic resistant primer (ResistantF) is in purple; the diagnostic susceptible primer (SusceptibleR) is in blue. Primers (SeqFPCR and SeqRPCR) for generating an 850 bp PCR product for sequencing are in orange; the primer utilized for sequencing (SequencingF) is in pink.