Table 1. Primers for kdr-like diagnostic PCR and Na_v gene fragments amplification and sequencing.

Name	Sequence (5’→3’)	Genome nucleotide positionΦ (VectorBase: CPIJ017894)	Purpose	Final conc. [nM]
ControlF	AAT CAG ACA ACG TGG ACC GCT TCC	A92919-C92896	Diagnostic PCR	125
ControlR	GTT TTT CGT TCC CAC CTT TTC TTG CAT GC	C92628-G92653	Diagnostic PCR	150
ResistantF	TTC TTG GCC ACC GTA GTG ATA GGA AAT TTT	T92758-A92729	Diagnostic PCR	150
SusceptibleR	TAA GAC TTC ACG CTG GAA TAC TCA CGA CT	A92701-A92729	Diagnostic PCR	250
SeqFPCR	CAT TCA CAC TGG CTT CCA ATT TCG ACT AG	C93037-G93009	Amplify PCR products to be sequenced	500
SeqRPCR	GGA CGC AAT CTG GCT TGT TAA CTT GTT T	G92188-T92215	Amplify PCR products to be sequenced	500
SequencingF	GAC AAC GTG GAC CGC TTC CCA A	G92914-C92895	Sequencing	5000

^Φ The reverse strand of the annotated gene, CPIJ017894, in the genome SuperContig 3.1170 in VectorBase encodes a partial sequence of the Cx. quinquefasciatus Na_v channel (sense strand) (see S1 Fig). The nucleotide positions shown here are as numbered in VectorBase.