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Effect of aggrecan, brevican, and tenascin-R on neurons in a 3D environment. A–J: Neurons were cultured for 1–14 days in vitro; example images are shown for day 1 and day 7 for all conditions. Neurons were fixed and stained for ßIII-tubulin (green) and DAPI (nucleus, blue). Each image represents a volume of 645 μm × 645 μm × 75 μm. Abbreviations: Aggr, aggrecan; Brev, brevican; TnR, tenascin-R; COLI, collagen I; DIV, days in vitro.
