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. 2020 Dec 3;5(23):e141670. doi: 10.1172/jci.insight.141670

Figure 4. RIIα-KO mice had decreased motivation for food rewards, sucrose solution intake, and sucrose preference compared with WT mice; differences were influenced by sex.

Figure 4

(A) Amount of time spent engaged in a progressive ratio (PR) operant task, (B) breakpoint, and (C) number of food pellets earned by WT and RIIα-KO littermates during PR operant task in fed and fasted states; n= 7–8/group, (female mice), analyzed by unpaired 2-way t tests. (D) Average daily intake of 10% sucrose solution of female and male RIIα-KO and WT mice; repeated-measure 2-way ANOVA with multiple comparisons (Bonferroni’s post hoc test) and (E) sucrose preference, measured as (daily sucrose solution intake [g]/total fluid intake [g]) × 100; sucrose intake and preference analyzed by repeated-measure 2-way ANOVA with multiple comparisons (Bonferroni’s post hoc test). (F) Cumulative total energy intake adjusted for body weight for female and male mice during 2-week experiment, measured as (sucrose kcal + chow kcal)/g BW, analyzed by 2-way unpaired t test (male) and Mann-Whitney nonparametric rank test (female). (G) Change in body weight during the 2-week sucrose intake experiment, analyzed by 2-way unpaired t test (male) and Mann-Whitney (female). For sucrose studies, n = 9–12/sex/group. All data are mean ± SEM; *P < 0.05; **P < 0.01, ****P < 0.0001.