Skip to main content
. 2020 Dec 3;5(23):e141670. doi: 10.1172/jci.insight.141670

Figure 6. Stereotaxic rAAV-mediated reexpression of Prkar2a rescued the sucrose and running phenotypes of RIIα-KO mice.

Figure 6

(A) Map of Prkar2a expression vector used to generate injectable rAAV and diagram of the injection strategy. (B) Representative image of coronal section from injected RIIα-KO mouse of a successful rAAV injection with expression of RIIα and GFP in MHb with magnified portion on right; scale bar represents 100 μm; for magnified portion, original magnification, 40×. (C) Sucrose intake and sucrose preference tests for rAAV-injected WT and KO mice revealed no differences between genotypes (n = 6–8/group; 7 males, 7 females), and (D) 2-week running wheel activity of rAAV-injected WT and KO mice did not differ between genotypes; (n = 7–8/group; 6 males, 9 females), repeated measure 2-way ANOVA with multiple comparisons (Bonferroni’s post hoc test) and a 2-tailed t test were used for sucrose and running analyses, respectively; all data represent the mean ± SEM.