Table 1.

Number and average area of individual adipocytes by method

	Cells counted (n)		Average area (µm2)
	Chow	High Fat Diet	Chow	High Fat Diet
Micrograph (manual)	208 ± 56	168 ± 35	N/A	N/A
Micrograph (Image J)	259 ± 129	155 ± 51	1280 ± 551	4987 ± 1450a
QuPath	2502 ± 1272	4511 ± 2863	2223 ± 640b	3320 ± 309a
Visiopharm	4633 ± 2047 c	6370 ± 3496	2367 ± 462b,c	3234 ± 373a,b,c

^ap-value <0.0001 compared to Chow average area of same method.

^bp-value <0.05 compared to Micrograph analysis of same diet.

^cp-value <0.05 compared to QuPath analysis of same diet.

Values are mean ± SD