Figure 1.
Diosmetin impairs the genomic stability of EC cells and inhibits the cell proliferation
(a): Effects of diosmetin on the proliferation of ISK, Hec-1B, AN3CA cells. Cells were seeded in 6-well plates at 2 × 105 cells (ISK, Hec-1B) or 3 × 105 cells (AN3CA) per well and treated by DMSO or diosmetin in indicated concentration for 72 hours, followed by cell number counting. Error bars represent SD. (b): Representative pictures of alkaline comet assay. Hec-1B cells were incubated with DMSO or diosmetin in indicated concentration for 72 hours and subjected to the comet assay. Scale bar, 100 μM. (c): Statistical analysis of alkaline comet assay. The tail moments of at least 50 cells were quantified using the Cometscore software (Sumerduck, VA, USA). Results were presented as mean ± SEM. Mann-Whitney U Test was used to compare the difference. ****: P < 0.0001.