Figure 3.

Diosmetin reduces the recruitment of RPA2

(a–c): Representative images of γH2AX (a), RPA2 (b), RAD51 (c) foci at different time points post IR (left) and statistical quantifications of the foci number (right). HCA2-hTERT cells were incubated with DMSO or diosmetin for 24 hours, then treated with X-ray (2 Gy). Cells were harvested immediately (0 h) or cultured for 2 h, 4 h, 16 h, 24 h before being harvested for immunofluorescence assay. Images were taken with a laser scanning confocal microscope (TCS SP8; Leica, Wetzlar, Germany). Scale bar, 10 μM. For each time point, at least 50 cells were counted. Error bars represent SEM.