Figure 4.

Diosmetin sensitizes EC cells to ionizing radiation

(a): Representative pictures of clonogenic assay manifesting viability of Hec-1B cells after diosmetin or IR treatment or both. (b): Statistical analysis of clonogenic assay. Hec-1B cells were pretreated with DMSO or diosmetin in indicated concentration for 24 hours, followed by irradiation in indicated dose. Then cells were continued to be treated with DMSO or diosmetin for 72 hours and incubated in the incubator until 2 weeks. Colonies with at least 50 cells were qualified. Analysis of nonlinear regression was used. The IC50 values were analyzed using a dose-effect analysis software. Error bars represent SD.