Figure 3.

ERK activation is required for G1/S transition in myogenic cells. (a) Experimental scheme of cre-mediated recombination and tissue clearing. Mice were injected with a MEK inhibitor (PD0325901, 5 mg/kg) at 2 and 2.5 dpi. MEK inhibitor-injected mice and uninjected controls were fixed at 3 dpi. (b–d) Representative images (b), averaged nuclear density (c), percentage (d) of myogenic cells expressing Fucci in mouse muscle regenerating regions. Magenta and green colors represent cells in G0/G1 and S/G2/M phase, respectively. All data above were obtained from the same experiment described in (a) (bars, SDs; N = 3 mice for each group; *p < 0.05)