Figure 1.

Characterization of 2D culture of HLE cells without FBS and effects of EW-7197 on tight junction molecules in 2D culture of HLE cells without FBS. (a) Phase-contrast and immunocytochemistry images for p63 and Ck7 in 2D culture of HLE cells without FBS. Scale bar, 20 μm. (b) RT-PCR for CLDN-1, −2, −4, −7, −18, LSR, TRIC and G3PDH (G3) in 2D-culture of HLE cells without FBS. M: 100 bp DNA ladder marker. (c) Immunocytochemistry for OCLN and LSR in 2D-culture of HLE cells (without FBS) treated with 10 μM EW-7197 for 24 h. (d) Western blotting for LSR, CLDN-1, −2, −4, −7, LSR, TRIC and actin in 2D culture of HLE cells (without FBS) treated with 10 μM EW-7197 for 24 h. The corresponding expression levels of D are shown as bar graphs