Figure 4.

Effects of p63 knockdown on epithelial permeability and tight junction molecules in 2.5D Matrigel culture of HLE cells. (a) Phase-contrast images and FD-4 assay in 2.5D Matrigel culture of HLE cells transfected with siRNA of p63 for 48 h and then pretreated with 10 μM EW-7197 2 h before treatment with 100 ng/ml HMGB1 for 24 h. Scale bar, 20 μm. (b) Western blotting for p63, LSR, CLDN-4 and actin in 2.5D Matrigel culture of HLE cells transfected with siRNA of p63 for 48 h and then pretreated with 10 μM EW-7197 2 h before treatment with 100 ng/ml HMGB1 for 24 h. The corresponding expression levels of B are shown as bar graphs