Figure 2.

Expression of miR-125b-5p in human primary moncytes and macrophages infected by H37Rv in vitro. Relative expression level of miR-125b-5p was measured with real-time PCR in (a) PBMCs from HC and TB patients, and (b, c) H37Rv-infected human peripheral blood monocytes-derived macrophages (MDMs) and THP-1 cells-derived macrophages (TDMs) for 48 h. (d) The mycobacterial viability in transfected MDMs and TDMs after H37Rv infection was determined by colony-forming units (CFU) assay. (e) FACS examined apoptosis rate of H37Rv-infected MDMs and TDMs. (f-h) Enzyme-linked immunosorbent assay (ELISA) measured levels of inflammatory factors, interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α in cell culture supernatant of H37Rv-infected MDMs and TDMs. (i) Western blot measured levels of apoptosis-related proteins, B cell lymphoma (Bcl)-2, Bcl-2-associated X Protein (Bax), and Bcl-2-interacting mediator (Bim) in H37Rv-infected MDMs and TDMs. All experiments were performed in triplicate and * P < 0.05