Figure 3.

Effect of miR-125b-5p blockage on human macrophage apoptosis in vitro with H37Rv infection. (a) Real-time PCR detected miR-125b-5p expression level in MDMs and TDMs transfected with miR-125b-5p inhibitor (in-miR-125b-5p) or its negative control (in-miR-NC). (bh) Above transfected MDMs and TDMs at 48 h were then subjected with H37Rv infection for 48 h. (b) Real-time PCR detected miR-125b-5p expression level. (c) CFU assay determined mycobacterial viability. (d) Apoptosis rate was recorded by FACS. (e–g) ELISA measured IL-6, IL-10 and TNF-α levels in cell culture supernatant. (h) Western blot assay examined Bcl-2, Bax and Bim levels with normalization to β-actin. All operations were carried out at least three times and * P < 0.05