Figure 5.

The promotion of miR-125b-5p knockdown on human macrophages apoptosis in vitro with H37Rv infection was abolished by silencing DRAM2. (a) Western blot assay examined expression level of DRAM2 in MDMs and TDMs transfected with siRNA against DRAM2 (si-DRAM2) or its negative control (si-NC). (bg) MDMs and TDMs were transfected with in-miR-125b-5p alone or combined with si-DRAM2 for 48 h, followed with H37Rv infection for 48 h. (b) CFU assay determined mycobacterial viability. (c) Apoptosis rate was recorded by FACS. (d–f) ELISA measured IL-6, IL-10, and TNF-α levels in cell culture supernatant. (g) Western blot assay examined Bcl-2, Bax, and Bim levels. All operations were launched in triplicate. * P < 0.05