Figure 4.

CircMTO1 served as a sponge of miR-199a-3p in gastric cell lines. (a) Starbase was used for the putative targeting site of circMTO1 and miR-199a-3p. (b) The bioactivity of luciferase analysis of AGS and HGC-27 cells was cotransfected with pmirglO-circMTO1-WT and pmirglO-circMTO1-Mut vector or miR-199a-3p mimics. (c) quantified RT-PCR data of miR-199a-3p with circMTO1 overexpression or knockdown. (d) quantified RT-PCR data of miR-199a-3p in the tissues of GC and nearby normal tissues (n = 68). (e) Pearson’s correlation analysis of miR-199a-3p and circMTO1 in GC tissues (n = 68, r = −0.5735, p < 0.001). Data are from three independent experiments. **p < 0.01, ***p < 0.001