Figure 1.

SNHG1 is highly expressed in patients with OPLL. A, PLL- or OPLL-derived LFCs identified by microscope observation and Vimentin immunofluorescence; B-C, mRNA (b) and protein (c) expression of osteogenesis-related factors BMP-2, RUNX2 and OCN determined by QRT-PCR and western blot analysis, respectively; D-E, the osteogenic properties of LFCs determined by Alizarin Red staining (d) and ALP (e) staining; F, the heatmap for differentially expressed lncRNAs between 4 OPLL tissues and 4 healthy PLL tissues determined by Arraystar Human LncRNA microarray V2.0 (Agilent_033010 Probe Name version); G, SNHG1 expression in 68 pairs of OPLL tissues and 18 pairs of PLL tissues identified by QRT-PCR; H, SNHG1 expression in cells after SNHG1 siRNA and SNHG1 overexpressing vector transfection determined by QRT-PCR as well. Data are expressed as the mean ± SD; in panels B and C, data were analyzed using two-way ANOVA, while data in panel H were analyzed using one-way ANOVA, and Tukey’s multiple comparisons test was used for the pairwise comparisons after ANOVA analysis; *, p < 0.05